LYNX Rapid HRP Antibody Conjugation Kit® LYNX Rapid HRP Antibody Conjugation Kit® LNK006Pbio-rad-产品中心-Avidity官网瑞孚迪Revvity和PerkinElmer珀金埃尔默，revvity瑞孚迪 (Revvity)为生命科学和诊断业务公司

LYNX Rapid HRP Antibody Conjugation Kit® LYNX Rapid HRP Antibody Conjugation Kit® LNK006Pbio-rad

2024-10-22

LYNX Rapid HRP Antibody Conjugation Kit®

货号：LNK006P,LNK005P,LNK004P,LNK003P,LNK002P,LNK001P

规格：3 Conjugations For 40µg Antibody,1 Conjugation For 20mg Antibody,5 Conjugations For 4mg Antibody,1 Conjugation For 4mg Antibody,3 Conjugations For 400µg Antibody,1 Conjugation For 400µg Antibody

市场价格：3128,19295,19890,6222,3868,2380

产品类型：流式抗体

品牌：Bio-Rad

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：HRP

产品介绍
LYNX Rapid HRP Antibody Conjugation Kit®enables the rapid conjugation of a pre-prepared lyophilized mixture containing Horseradish peroxidase (HRP) label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in directional covalent bonding of HRP to the antibody.The LYNX Rapid Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.
产品详情
Reagents in the Kit	Pack Size: 1 Conjugation For 400µg Antibody1 Vial of 100μg LYNX lyophilized HRP mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagentPack Size: 3 Conjugations For 400µg Antibody3 Vials of 100ug LYNX lyophilized HRP mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagentPack Size: 1 Conjugation For 4mg Antibody1 Vial of 1mg LYNX lyophilized HRP mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagentPack Size: 5 Conjugations For 4mg Antibody5 Vials of 1mg LYNX lyophilized HRP mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagentPack Size: 1 Conjugation For 20mg Antibody1 Vial of 5mg LYNX lyophilized HRP mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagentPack Size: 3 Conjugations For 40µg Antibody3 Vials of 10μg LYNX lyophilized HRP mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagent
Preparing The Antibody	Pack Size: 1 Conjugation For 400µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 100μg HRP you need to add between 100-400μg of antibody. For optimal results the antibody volume should be up to 100μl, at a concentration range of 0.5-5.0mg/ml.Pack Size: 3 Conjugations For 400µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 100μg HRP you need to add between 100-400μg of antibody. For optimal results the antibody volume should be up to 100μl, at a concentration range of 0.5-5.0mg/ml.Pack Size: 1 Conjugation For 4mg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 1mg HRP you need to add between 1-4mg of antibody.. For optimal results the antibody volume should be up to 1ml, at a concentration range of 0.5-5.0mg/ml.Pack Size: 5 Conjugations For 4mg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 4mg HRP you need to add between 1-4mg of antibody. For optimal results the antibody volume should be up to 1ml, at a concentration range of 0.5-5.0mg/ml.Pack Size: 1 Conjugation For 20mg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 5mg HRP you need to add between 5-20mg of antibody. For optimal results the antibody volume should be up to 5ml, at a concentration range of 0.5-5.0mg/ml.Pack Size: 3 Conjugations For 40µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.Sodium azide is an irreversible inhibitor of HRP and therefore should be avoided.The amount of antibody used for labeling ideally should correspond to molar ratios between 1:4 and 1:1 Ab to HRP. Taking account of the molecular weights (160,000 versus 40,000), this means for that for 10μg HRP you need to add between 10-40μg of antibody. For optimal results the antibody volume should be up to 10μl, at a concentration range of 0.5-5.0mg/ml.
Regulatory	For research purposes only
Guarantee	Guaranteed until date of expiry. Please see product label.
存储条件
This kit contains lyophilized hygroscopic components that are moisture-sensitive. This kit is shipped under ambient conditions with silica packets to avoid exposure to moisture. On receipt, Bio-Rad recommend that the kit is stored at -20oC and protected from moisture. Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing. Before opening, allow the components to reach room temperature to minimize condensation.
应用
Application Name	Verified	Min Dilution	Max Dilution
Conjugation	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. Instructions For Use 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25°C) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.

技术参数

产品类型 Conjugation Kit

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