Alk. Phos Conjugation Kit Alk. Phos Conjugation Kit bio-rad LNK011AP-产品中心-Avidity官网瑞孚迪Revvity和PerkinElmer珀金埃尔默，revvity瑞孚迪 (Revvity)为生命科学和诊断业务公司

Alk. Phos Conjugation Kit Alk. Phos Conjugation Kit bio-rad LNK011AP

2024-10-22

Alk. Phos Conjugation Kit

货号：LNK011AP,LNK012AP,LNK013AP,LNK014AP

规格：1 Conjugation For 100µg Antibody,3 Conjugations For 100µg Antibody,1 Conjugation For 1mg Antibody ,3 Conjugations For 10µg Antibody

市场价格：2788,5015,7123,3128

产品类型：抗体和染料

品牌：Bio-Rad

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：AP(碱性磷酸酶)

产品介绍
LYNX Rapid Alkaline Phosphatase Antibody Conjugation Kit®enables the rapid conjugation of a pre-prepared lyophilized mixture containing Alkaline Phosphatase (AP) label to an antibody or protein. Activation of proprietary reagents within the antibody-label solution results in directional covalent bonding of AP to the antibody.The LYNX Rapid Conjugation kit® can be used to label small quantities of antibody/protein at near neutral pH, allowing a high conjugation efficiency with 100% antibody recovery.
产品详情
Reagents in the Kit	Pack Size: 1 Conjugation For 100µg Antibody1 Vial of 100ug LYNX lyophilized AP mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagentPack Size: 3 Conjugations For 100µg Antibody3 Vials of 100ug LYNX lyophilized AP mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagentPack Size: 1 Conjugation For 1mg Antibody1 Vial of 1mg LYNX lyophilized AP mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagentPack Size: 3 Conjugations For 10µg Antibody3 Vials of 10ug LYNX lyophilized AP mix1 Vial LYNX Modifier reagent1 Vial LYNX Quencher reagent
Preparing The Antibody	Pack Size: 1 Conjugation For 100µg Antibody, 3 Conjugations For 100µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.The molar ratio of antibody: AP should be 1:1, i.e. 100ug antibody to every 100ug AP. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 100ul and a maximum antibody amount of 100ug. Antibody at a concentration of greater than 1mg/ml requires dilution. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit. Phosphate may inhibit alkaline phosphatase activity. This kit can be used with antibodies prepared in phosphate buffer as long as the buffer will be washed away during an immunoassay procedure. Otherwise we recommend using a non-phosphate containing buffer for labeling antibodies with alkaline phosphatase.Pack Size: 1 Conjugation For 1mg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.The molar ratio of antibody: AP should be 1:1, i.e. 1mg antibody to every 1mg AP. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 1ml and a maximum antibody amount of 1mg. Antibody at a concentration of greater than 1mg/ml requires dilution. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit. Phosphate may inhibit alkaline phosphatase activity. This kit can be used with antibodies prepared in phosphate buffer as long as the buffer will be washed away during an immunoassay procedure. Otherwise we recommend using a non-phosphate containing buffer for labeling antibodies with alkaline phosphatase.Pack Size: 3 Conjugations For 10µg AntibodyThe following buffer solutions are recommended for preparing the antibody:10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.The molar ratio of antibody: AP should be 1:1, i.e. 10ug antibody to every 10ug AP. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 10ul and a maximum antibody amount of 10ug. Antibody at a concentration of greater than 1mg/ml requires dilution. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit. Phosphate may inhibit alkaline phosphatase activity. This kit can be used with antibodies prepared in phosphate buffer as long as the buffer will be washed away during an immunoassay procedure. Otherwise we recommend using a non-phosphate containing buffer for labeling antibodies with alkaline phosphatase.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This kit contains lyophilized hygroscopic components that are moisture-sensitive. This kit is shipped under ambient conditions with silica packets to avoid exposure to moisture. On receipt, Bio-Rad recommend that the kit is stored at -20oC and protected from moisture. Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing. Before opening, allow the components to reach room temperature to minimize condensation.
应用
Application Name	Verified	Min Dilution	Max Dilution
Conjugation	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage. Instructions For Use 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25°C) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1ul of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1ul of Quencher reagent for every 10ul of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10ul of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use. 1. To the antibody sample add 1μl of the Modifier reagent for every 10μl of antibody and mix gently.2. Pipette the mixed antibody-modifier sample directly onto the LYNX lyophilized mix and gently pipette up and down twice to resuspend.3. Replace cap onto vial and incubate at room temperature (20-25oC) for 3 hours, or overnight if preferred.4. After incubation, add 1μl of Quencher reagent for every 10μl of antibody used. Leave to stand for 30 minutes before use.

技术参数

产品应用 wb

产品类型 Polyclonal Antibody

推荐用于 wb

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